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United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
or policies of any United States Government agency.
This document is provided "as is", without warranty of any kind,
express or implied, including but not limited to the warranties
of merchantability, fitness for a particular purpose and
non-infringement. In no event shall the authors be liable
for any claim, damages or other liability, whether in an
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or in connection with the document or the use or other dealings
made with the document.
PRINCIPLE OF THE TEST.
The Anatomic Pathology Laboratory is responsible for processing all tissues
obtained at surgery, outpatient clinics, and postmortems in this hospital.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515),
a Gynecologic Cytology Form
(U. S. Standard Form 541, USSF541),
or a filled-out Authorization for Autopsy
(U. S. Standard Form 523, USSF523).
REAGENTS, INSTRUMENTATION.
Plastic containers.
Tissue Tek VIP® Vacuum Infiltration Processor.
Neutral 10% buffered formalin.
70% Flex Solution.
80% Flex Solution.
95% Flex Solution.
100% Flex Solution.
Clearing Solution.
Paraffin.
Decalcifying solution (RDO),
Hematoxylin and eosin stain.
Microtome.
Water bath.
Alcoholic iodine solution.
5% sodium thiosulfate.
13% Thioacetamide.
Fluted filter paper.
STEP-BY-STEP DESCRIPTION.
Surgical Pathology specimens are received in the Histology Laboratory
from different areas of the hospital, as follows:
1. Operating Room: Surgery Service.
2. Genitourinary Service.
3. Dermatology Service.
4. Radiology Service.
5. Dental Service.
6. Podiatry Service.
7. Hospital wards.
8. Gastroenterology Service.
9. Cat Scan.
10. Outpatient areas, including Women's clinic.
All specimens are received in 10% buffered formalin except:
1. Fresh tissue.
a. Frozen Sections: Rush diagnosis. (Intra-operative consultation).
b. Tumor Collections: e.g., lungs, colons, pancreas, esophagus, prostate
c. Fresh lymph nodes.
REFERENCES.
1. Berte LM, Charlton BJ, Kirkley B, Schiffgens J,
Wilson JI, Woodcock SM.
Clinical Laboratory Technical Procedure Manuals;
Approved Guideline -- Fourth Edition.
NCCLS Document GP2-A4. 2002;2(5):.
ISBN 1-56238-458-9, 64 pages.
National Committee for Clinical Laboratory Standards (NCCLS).
940 West Valley Road, Suite 1400. Wayne, PA 19087-1898.
... presents the important components of writing and managing procedures
for the clinical laboratory.
2. Hoeltge GA, Dynek DA, Delahunty DC, McClatchey KD,
Rabinovitch A, Robinowitz M, Travers EM.
National Committee for Clinical Laboratory Standards (NCCLS).
Clinical Laboratory Technical Procedure Manuals. Third Edition.
Approved Guideline GP2-A3. 1996;16(15):.
3. Rosai J.
Rosai and Ackerman's Surgical Pathology. Ninth Edition.
St Louis: C.V. Mosby. 2004;:.
ISBN: 0323013422, 3080 pages.
4. Mills SE, Carter D, Greenson JK, Oberman HA,
Reuter VE, Stoler MH, eds.
Sternberg's Diagnostic Surgical Pathology. Fourth Edition.
New York: Lippincott Williams & Wilkins. 2004;:.
ISBN: 0781740517, 3089 pages.