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United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
or policies of any United States Government agency.
This document is provided "as is", without warranty of any kind,
express or implied, including but not limited to the warranties
of merchantability, fitness for a particular purpose and
non-infringement. In no event shall the authors be liable
for any claim, damages or other liability, whether in an
action of contract, tort or otherwise, arising from, out of,
or in connection with the document or the use or other dealings
made with the document.
See also: Main objectives: 1.
PRINCIPLE OF THE TEST.
Proper examination and accurate description of specimens must be performed,
for optimal clinicopathology correlation, hence optimal patient care.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
Plastic cassettes.
Tissue-Tek pencil.
Ink.
Applicator.
Clean dissection instruments.
Cassette recorder and tape.
Microscope.
STEP-BY-STEP DESCRIPTION.
1. After proper registration of the specimen, the requisition and specimen
in its original container are transferred to specimen bench.
2. A plastic cassette is prepared with the full specimen log number written
with the tissue tek pencil on the smooth surface. Specimens separately
received from the same patient have a number after the surgical number
of all specimens (e.g. 74-1000-1). The subcoding of the large specimens
is part of the examination, and is at the discretion and direction of the
pathology resident.
3. When specimens and cassettes are consecutively arranged and the
requisition slips are in order, the examining resident is so informed.
4. Clean dissection instruments are placed daily in the surgical desk.
After use, they are soaked 20 minutes in surgical disinfectant and rinsed.
Their maintenance is the responsibility of the Histology Laboratory,
and periodically their number and condition are examined; scissors in
particular, require periodic sharpening and tightening. A special
container for used scalpel blades must be labeled and properly disposed
of as "contaminated material" in RED BIOHAZARD BAGS.
Surgical implements carry the danger
of hepatitis and constant vigilance is required to avoid this danger.
Any significant skin injury associated with their use should be pursued
with medical examination and reporting.
5. All gross specimens are examined exclusively by resident
pathologists or by attending pathologists. Resident pathologists
are supervised by attending pathologists.
A gross dissection procedure manual has been prepared for referral
by the resident pathologists and others. The procedure manual
is kept in the histology laboratory, room 4D-124.
5.1. Gross descriptions must be clear, concise and to contain adequate
information regarding type, size and/or weight of specimens,
measurements, and extent of gross lesions.
5.2. All gross descriptions include a section key at the end of the
gross note. This section key includes block and slide designations for
sections, explaining any specific significance of each section (e.g.,
margins of resection, deepest penetration of tumor, breast quadrants,
lymph node levels, etc.)
5.3. The attending pathologist reviews the gross description for
completeness, and is responsible for the completeness of the final report.
The attending pathologist also assures that the pathologic diagnosis relates
sensibly with the history provided by the clinician, the post-operative
diagnosis provided by the clinician, and the gross description provided by
the resident pathologist. Cases where the microscopic diagnosis does not
correspond with the post-operative diagnosis provided by the clinicians
become cases for review by the Invasive Procedures Review Committee
(IPRC, formerly SCRC). In order to assure that the case is reviewed
by the IPRC, the attending pathologist who signs out the case must
SNOMED-code the case under the Procedure axis with the code term SCRC.
This SNOMED-code is picked up by a monthly search for new IPRC cases
by the Pathology representative for the IPRC who prepares
the pathology monitor. In most cases, a "Microscopic description"
is not included as part of the surgical pathology report.
Instead, the pathologists use a "Comment" section in which any information
of clinical, pathologic or administrative interest (e.g.,
documentation that the clinician was notified of a new "positive"
diagnosis other than basal cell carcinoma) is included.
5.4. The COMMENT section also must include the results of special stains
or ancillary studies (e.g., immunohistochemistry, nucleic acid probes,
cytogenetics) with the morphologic diagnosis. When the results of
special studies come in after the case has been released, the pathologist
issues a SUPPLEMENTAL REPORT, which the VISTA Computer System automatically
adds on to the permanent copy of the report kept in the computer files.
In addition, a typed signed copy of the entire report including
the Supplemental report is issued. If the Supplemental report
contains information that is of direct clinical importance
(i.e., that may change treatment), then the attending pathologist notifies
the clinician and documents the notification in the Supplemental report.
5.5. The final reports of tumor diagnoses always provides sufficient
information as to the grade of tumor and extent of disease. We
generally do not use clinical staging terminology in our reports,
preferring to provide all the pathology information that the clinicians
would need to stage their patient for use in standard systems of
grading. We feel this is important to make sure that our diagnoses
stand against changes and modifications introduced to staging
classifications.
5.6.
The exceptions consist of cases that are being prepared for the
cancer evaluation program of the American College of Surgeons.
See Procedure 141: CAP ANATOMIC PATHOLOGY
REPORTING PROTOCOLS. For these reports, TNM staging should
be reported, as indicated in the protocols for individual organs.
5.7. After the Resident's examination and description,
specimens are placed in cassettes, and then into formalin.
Small specimens (less than 0.5 cm) are wrapped in teabags,
or blue sponges, before they are put in cassettes.
5.8. All specimens are submitted for microscopic examination, except
for amputations due to peripheral vascular disease;
degenerative joint disease; bunions; and hernia sacs;
and metal or plastic prosthetic devices.
5.9. The laboratory does not accept tissues or other materials
that may contain radioactive material.
6. Any residual tissue remaining after sections are submitted is retained
until after the final diagnosis is rendered. Small specimens are retained
in their original container. Covered buckets with formalin are used for
larger specimens. All specimens are labeled with surgical number, and stored
consecutively in storage cabinets. Periodically, usually every month,
the attending pathologist examines the surgical logbook, marking specimens
for permanent retention. Other specimens are discarded
by Histology personnel.
7. At the end of each working day, the resident places
the dictation paperwork and the dictation cassettes
on the multi-headed microscope table in the Resident's Office,
4D-146. These materials are picked up in the morning
by the clerk-typist, for transcription.
8. Examination area and instruments are cleaned.
9. Baskets in formalin buckets are transferred to the Tissue-Tek®
VIP® 5 Vacuum Infiltration Processor at the end of the day.
10. Facilities are available in pathology, as well as in surgery and other
clinical departments, for imaging directly into the VistA® computer
system. The cameras in pathology are connected to photomicroscopes.
The MEDICAL MEDIA SERVICE has responsibility
for capturing images of gross specimens received by pathology.
In the event that Medical Media is unavailable (i.e., after hours),
the resident may take gross photographs using the small camera
in the top right-hand drawer near the microscope
in the Anatomic Pathology sign-out room, 4D-139.
Other departments have facilities for capturing gross images
of their specimens as well as endoscopic images,
images of the patient, and in-situ lesions.
11. Images can be downloaded as external files by
MEDICAL MEDIA.
REFERENCES.
1. Prophet EB, Mills B, Arrington JB, Sobin LH.
Laboratory Methods in Histotechnology, pp. 33-38.
Armed Forces Institute of Pathology.
Washington, DC. 20306-6000: Armed Forces Institute of Pathology.
ISBN: 1-881041-00-X 1992;:33-38.
2. Berte LM, Charlton BJ, Kirkley B, Schiffgens J,
Wilson JI, Woodcock SM.
Clinical Laboratory Technical Procedure Manuals;
Approved Guideline -- Fourth Edition.
NCCLS Document GP2-A4. 2002;2(5):.
ISBN 1-56238-458-9, 64 pages.
National Committee for Clinical Laboratory Standards (NCCLS).
940 West Valley Road, Suite 1400. Wayne, PA 19087-1898.
... presents the important components of writing and managing procedures
for the clinical laboratory.
3. Hoeltge GA, Dynek DA, Delahunty DC, McClatchey KD,
Rabinovitch A, Robinowitz M, Travers EM.
National Committee for Clinical Laboratory Standards (NCCLS).
Clinical Laboratory Technical Procedure Manuals. Third Edition.
Approved Guideline GP2-A3. 1996;16(15):.
4. Rosai J.
Rosai and Ackerman's Surgical Pathology. Ninth Edition.
St Louis: C.V. Mosby. 2004;:.
ISBN: 0323013422, 3080 pages.
5. Mills SE, Carter D, Greenson JK, Oberman HA,
Reuter VE, Stoler MH, eds.
Sternberg's Diagnostic Surgical Pathology. Fourth Edition.
New York: Lippincott Williams & Wilkins. 2004;:.
ISBN: 0781740517, 3089 pages.
6. Westra WH, Hruban RH, Phelps TH, Isacson C.
Surgical Pathology Dissection. An Illustrated Guide.
Second Edition. With a Forward by Askin FB.
New York: Springer. 2002.
ISBN 0-387-95559-3, 258 pages.
7. Lester SC.
Manual of Surgical Pathology.
New York: Churchill Livingstone.
A Harcourt Health Sciences Company. 2001.
ISBN 0-443-07918-8, 336 pages.
8. Sakura Tissue-Tek® VIP® 5 Vacuum Infiltration Processor.
Operating Manual.
© 2001. Sakura Finitek, U.S.A., Inc.
Technical Support: 1-800-543-8496.
Sakura Finitek, U.S.A., Inc. © 2001.
1750 W. 214th Street
Torrance, CA 90501