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United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
or policies of any United States Government agency.
This document is provided "as is", without warranty of any kind,
express or implied, including but not limited to the warranties
of merchantability, fitness for a particular purpose and
non-infringement. In no event shall the authors be liable
for any claim, damages or other liability, whether in an
action of contract, tort or otherwise, arising from, out of,
or in connection with the document or the use or other dealings
made with the document.
See also: Main objectives: 1.
PRINCIPLE OF THE TEST.
Proper fixation of tissues must be performed
for optimal histologic sections, that are necessary
for accurate diagnosis. The exclusive fixative recommended
in surgical pathology is neutral 10% buffered formalin.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics,
and postmortems, fresh or in fixative, along with a filled-out
Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
Neutral 10% buffered formalin. Plastic containers.
STEP-BY-STEP DESCRIPTION.
1. The exclusive fixative recommended in surgical pathology
is neutral 10% buffered formalin, which is purchased
in five gallon containers as a prepared reagent.
2. 4F1G is used for electron microscopic examination,
and it is received as a prepared reagent.
REFERENCE.
1. Prophet EB, Mills B, Arrington JB, Sobin LH.
Laboratory Methods in Histotechnology, pp. 25-28.
1992: Armed Forces Institute of Pathology,
Washington, DC. 20306-6000.
ISBN: 1-881041-00-X 1992.