DAILY TIME SCHEDULE FOR
TISSUE-TEK VIP®5
VACUUM INFILTRATION PROCESSOR.
DRAFT COPY ONLY.
(Procedure 13).
http://www.netautopsy.org/axsop/axsop013.htm


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United States Government Work, uncopyrighted, public-domain, DRAFT COPY ONLY. This document does not necessarily represent the views or policies of any United States Government agency. This document is provided "as is", without warranty of any kind, express or implied, including but not limited to the warranties of merchantability, fitness for a particular purpose and non-infringement. In no event shall the authors be liable for any claim, damages or other liability, whether in an action of contract, tort or otherwise, arising from, out of, or in connection with the document or the use or other dealings made with the document.

See also: Tissue-Tek® Processor: 11, 12, 14.



PRINCIPLE OF THE TEST.
      Schedule of operation for the tissue processor.



SPECIMEN REQUIRED.

All human tissue excised at surgery, outpatient clinics, and postmortems, fresh or in fixative, along with a filled-out Tissue Examination Form (U. S. Standard Form 515, USSF515).



REAGENTS, INSTRUMENTATION.

10% Neutral Buffered Formalin. 70% Flex solution. 80% Flex solution. 95% Flex solution. 100% Flex solution. Clearing solution. Paraffin.



STEP-BY-STEP DESCRIPTION.
      Tissue stays in first station until 7 o'clock. 
STATIONS       SOLUTIONS    CONC         TIMES      TEMP     P/V     MIX
  1            Formalin     10%          55min       off     off     off
  2            Formalin     10%          55min        -                 
  3            Alcohol      70%          55min                          
  4            Alcohol      80%          55min        -                 
  5            Alcohol      95%          55min         
  6            Alcohol      95%          55min         
  7            Alcohol     100%          55min          
  8            Alcohol     100%          55min          
  9            Clearant                  50min          
 10            Clearant                  40min          
 11            Paraffin                  25min        60
 12            Paraffin                  25min        60
 13            Paraffin                  55min        60
 14            Paraffin                  00min        60




REFERENCE.

1. Sakura Tissue-Tek® VIP® 5 Vacuum Infiltration Processor. Operating Manual.
© 2001. Sakura Finitek, U.S.A., Inc.
Technical Support: 1-800-543-8496.
Sakura Finitek, U.S.A., Inc. © 2001.
1750 W. 214th Street
Torrance, CA 90501