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DRAFT COPY ONLY. This document does not necessarily represent the views
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PRINCIPLE OF THE TEST.
Proper maintenance of the microtome is necessary for
optimal histologic sections.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out
Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
Microtome. Lubricant.
STEP-BY-STEP DESCRIPTION.
Figure 1. Embedding Center.
See also: 16,
39.
Figure 2. Refrigerator.
See also: 39.
Figure 3. Frozen Section Staining.
See also: 24,
25, 26,
27, 39,
41.
Figure 4. Shandon Staining.
See also: 39.
Figure 5. H&E Staining Table.
See also: 39.
Figure 6. Grossing Station.
See also: 39.
Figure 7. Paraffin Dispenser.
See also: 39.
Figure 8. Tissue-Tek Processor.
See also: 14,
15, 16,
17.
Figure 9. Cryostat.
See also: 39,
40.
Figure 10. pH Monitor.
See also: 39,
85.
Figure 11. Microwave.
See also: 23,
39.
Figure 12. Microscope.
See also: 39,
117.
Figure 13. Microscope in Signout Room.
See also: 39,
117.
Figure 20. Microtome and Waterbath Maintenance Sheet.
Figure 21. VAMHCS Equipment Maintenance Log. Tissue Processor.
Figure 22. VAMHCS Equipment Maintenance. Cryostat. Tissue Tek.
Figure 23. Autostaining. Shandon.
Figure 24. Incubation.
Figure 25. Refrigeration.
Figure 26. Embedding Center.
Figure 27. VAMHCS Equipment Maintenance. Kenmore Microwave.
Figure 28. VAMHCS Equipment Maintenance. Grossing Station. 65304395.
Figure 29. Special Stain Area.
Figure 30. VAMHCS Frozen Section Staining Log.
Figure 31. VAMHCS Equipment Maintenance.
TBS Paraffin Dispenser 9506X0127-05.
Figure 32. Laboratory Eyewash Flush.
Figure 33. Weekly Microscope Maintenance Log.
Figure 34. Weekly Double-headed Frozen-section Microscope Maintenance Log.
Figure 35.0. Autopsy Template. Anterior.
Figure 35.1. Autopsy Template. Posterior.
REFERENCE.
1. Prophet EB, Mills B, Arrington JB, Sobin LH.
Laboratory Methods in Histotechnology.
Armed Forces Institute of Pathology, Washington, DC. 20306-6000.
1992;:45-52.
ISBN: 1-881041-00-X 1992.