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United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
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PRINCIPLE OF THE TEST.
Proper processing of bone marrow tissue
for optimal histologic sections and aspiration slides.
SPECIMEN REQUIRED.
All human bone marrow tissue excised at surgery, outpatient clinics,
and postmortems, fresh or in fixative, along with a filled-out
Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
Bouin's fixative.
10% neutral buffered formalin.
Alcohol.
STEP-BY-STEP DESCRIPTION.
1. If bone marrow aspirates and biopsies arrive already in Bouin's
fixative, allow to remain overnight. The preferred fixative is 10% neutral
buffered formalin.
2. The aspirate is poured into a teabag. The container is rinsed
with 70% alcohol which is then poured into the teabag to assure that all
the aspirate is submitted for processing. When the fluid has drained,
fold the bag so that it fits into a cassette.
3. The biopsy is washed in 70% alcohol for 30 minutes then placed
in decal II solution for 30 minutes. It is then put into a cassette (yellow)
and washed with the aspirate.
4. The biopsy and the aspirate should wash in 70% alcohol with
constant agitation for 6 - 7 hours before being put on the processor.
This is accomplished by putting the cassettes into the container with
a stirrer on the stir plate.
REFERENCE.
1. Prophet EB, Mills B, Arrington JB, Sobin LH.
Laboratory Methods in Histotechnology, pp. 45-52.
1992: Armed Forces Institute of Pathology,
Washington, DC. 20306-6000.
ISBN: 1-881041-00-X 1992.