STAINING QUALITY IN HISTOLOGY
DRAFT COPY ONLY.
(Procedure 34).
http://www.netautopsy.org/axsop/axsop034.htm


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United States Government Work, uncopyrighted, public-domain, DRAFT COPY ONLY. This document does not necessarily represent the views or policies of any United States Government agency. This document is provided "as is", without warranty of any kind, express or implied, including but not limited to the warranties of merchantability, fitness for a particular purpose and non-infringement. In no event shall the authors be liable for any claim, damages or other liability, whether in an action of contract, tort or otherwise, arising from, out of, or in connection with the document or the use or other dealings made with the document.

See also: Quality improvement program: 33, 35, 113, 137, 138.



PRINCIPLE OF THE TEST.


All surgical pathology cases are reviewed by the pathologists for standards of quality.



SPECIMEN REQUIRED.


All human tissue excised at surgery, outpatient clinics, and postmortems, fresh or in fixative, along with a filled-out Tissue Examination Form (U. S. Standard Form 515, USSF515).



REAGENTS, INSTRUMENTATION.


Hematoxylin and eosin stains. Special stains.



STEP-BY-STEP DESCRIPTION.


1. Each day, all surgical pathology cases are reviewed by the pathologists at the QA conference. The adequacy of staining (frozen sections and permanent sections) is assessed, and documented in the signed logbook in the Anatomic Pathology Signout Room, 4D-139.

2. For special stains, control slides are always included and examined. ALL STAINS are checked for their intended reactivity at the time of use, AND INITIALED IN THE HISTOLOGY LOGBOOK BY THE PATHOLOGIST. If there are any deficiencies, a note is written in the QA logbook.

3. The supervisor is notified any problem by voice and by e-mail by the CHIEF, SURGICAL PATHOLOGY SECTION. A note of the deficiency is appended to the histotechnologist's performance record.



REFERENCE.


1. Rosai J.
Rosai and Ackerman's Surgical Pathology. Ninth Edition.
St Louis: C.V. Mosby. 2004;:.
ISBN: 0323013422, 3080 pages.

2. Mills SE, Carter D, Greenson JK, Oberman HA, Reuter VE, Stoler MH, eds.
Sternberg's Diagnostic Surgical Pathology. Fourth Edition.
New York: Lippincott Williams & Wilkins. 2004;:.
ISBN: 0781740517, 3089 pages.