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United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
or policies of any United States Government agency.
This document is provided "as is", without warranty of any kind,
express or implied, including but not limited to the warranties
of merchantability, fitness for a particular purpose and
non-infringement. In no event shall the authors be liable
for any claim, damages or other liability, whether in an
action of contract, tort or otherwise, arising from, out of,
or in connection with the document or the use or other dealings
made with the document.
See also: Maintenance policies: 39,
41, 43,
44, 46,
117.
PRINCIPLE OF THE TEST.
The cryostat is used and maintained daily
for optimal performance.
SPECIMEN REQUIRED.
All fresh human tissue excised at surgery,
outpatient clinics, and postmortems,
along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
Cryostat.
Flex or acetone.
STEP-BY-STEP DESCRIPTION.
The cryostat is used daily and is washed out daily with 100% Flex.
Procedure for decontamination of cryostats when frozen section has
been completed on tissue tagged for tuberculosis or other infectious disease.
Cryostat #1 Laboratory Technician.
1. Dial should be turned to defrost.
2. After defrosting, any debris collected in bag should
be disposed in a contaminated material bag (orange).
3. Cryostat chamber should be washed down with bleach and Flex 100%.
4. Microtome will be removed at this time and washed with 2% Amphyl.
5. Allow to dry thoroughly overnight.
6. Squirt 100% flex or acetone in any spaces where water
may be trapped.
7. Follow with procedure of oiling according to manual for cryostats
which is placed in Frozen Section Room.
Cryostat #2 (Reichert Cryostat) use as back up.
The same procedure should be followed for this instrument.
Dissecting instruments should be decontaminated with 2% Amphyl.
REFERENCE.
1. Prophet EB, Mills B, Arrington JB, Sobin LH.
Laboratory Methods in Histotechnology, pp. 67-70.
1992;:. Armed Forces Institute of Pathology,
Washington, DC. 20306-6000.
ISBN: 1-881041-00-X 1992.