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PRINCIPLE OF THE TEST.
Chromogen used in immunohistochemistry for optimal antigen detection.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
25 mg DAB.
100 ml Tris buffer, pH 7.6.
45 micron filter.
1 ml of 3% hydrogen peroxide solution.
STEP-BY-STEP DESCRIPTION.
1. Dissolve 25 mg DAB in 100 ml Tris buffer pH 7.6.
2. Filter with a 45 micron filter.
3. Add 1 ml of a 3% hydrogen peroxide solution.
4. Pour into a Coplin jar containing your slides.
REFERENCES.
1. Miller KD.
Immunohistochemical Techniques. Chapter 21. pp. 421-464.
In: Bancroft JD, Gamble M.
Theory and Practice of Histological Techniques. Fifth Edition.
Edinburgh: Churchill Livingstone. 2002;:421-464.
ISBN 0-443-06435-0, 796 pages.
DAB = 3.3'-diaminobenzidine tetrahydrochloride
(substrate for peroxidase methods).
2. Prophet EB, Mills B, Arrington JB, Sobin LH, eds.
Laboratory Methods in Histotechnology.
Washington, DC: Armed Forces Institute of Pathology. 1992;:.
ISBN 1-881041-00-X, 278 pages.
3. Mikel UV, ed.
Advanced Laboratory Methods in Histology and Pathology.
Washington, DC: Armed Forces Institute of Pathology. 1994;:.
ISBN 1-881041-13-1, 254 pages.