NEXT PAGE
PREVIOUS PAGE
RETURN TO TABLE OF CONTENTS
United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
or policies of any United States Government agency.
This document is provided "as is", without warranty of any kind,
express or implied, including but not limited to the warranties
of merchantability, fitness for a particular purpose and
non-infringement. In no event shall the authors be liable
for any claim, damages or other liability, whether in an
action of contract, tort or otherwise, arising from, out of,
or in connection with the document or the use or other dealings
made with the document.
PRINCIPLE OF THE TEST.
Alternative chromogen used in immunohistochemistry,
for precipitation other than brown color (namely, red),
for optimal results.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
4 mg of AEC.
1 ml N,N dimethylformamide.
14 ml of 0.1% sodium acetate buffer, pH 5.2.
0.15 ml 3% hydrogen peroxide.
45 micron filter.
STEP-BY-STEP DESCRIPTION.
1. Dissolve 4 mg of AEC in 1 ml N,N dimethylformamide.
2. Add 14 ml of 0.1% sodium acetate buffer pH 5.2.
3. Add 0.15 ml 3% hydrogen peroxide.
4. Filter with 45 micron filter.
REFERENCES.
1. Miller KD.
Immunohistochemical Techniques.
Chapter 21. pp. 421-464.
In: Bancroft JD, Gamble M.
Theory and Practice of Histological Techniques. Fifth Edition.
Edinburgh: Churchill Livingstone. 2002;:421-464.
ISBN 0-443-06435-0, 796 pages.
AEC = 3-amino-9-ethylcarbazole
(substrate for peroxidase methods).
2. Prophet EB, Mills B, Arrington JB, Sobin LH, eds.
Laboratory Methods in Histotechnology.
Washington, DC: Armed Forces Institute of Pathology. 1992;:.
ISBN 1-881041-00-X, 278 pages.
3. Mikel UV, ed.
Advanced Laboratory Methods in Histology and Pathology.
Washington, DC: Armed Forces Institute of Pathology. 1994;:.
ISBN 1-881041-13-1, 254 pages.