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PRINCIPLE OF THE TEST.
Used in immunohistochemistry for optimal histologic sections.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
0.5% polylysine solution.
Polylysine (Sigma).
STEP-BY-STEP DESCRIPTION.
1. SOLUTION:
0.5% polylysine in distilled water.
0.05 grams of polylysine.
50 ml of distilled water.
2. PROCEDURE:
Coat slides by rubbing the unfrosted BreB together as you would
do when making blood smears. Do not use your fingers,
as you will produce a keratin layer.
SOURCE OF POLYLYSINE: Sigma Chemicals.
REFERENCES.
1. Anderson G, Bancroft J.
Tissue Processing and Microtomy including Frozen. Chapter 6, pp. 85-107.
In: Bancroft JD, Gamble M.
Theory and Practice of Histological Techniques. Fifth Edition.
Edinburgh: Churchill Livingstone. 2002;:85-107.
ISBN 0-443-06435-0, 796 pages.
2. Prophet EB, Mills B, Arrington JB, Sobin LH, eds.
Laboratory Methods in Histotechnology.
Washington, DC: Armed Forces Institute of Pathology. 1992;:.
ISBN 1-881041-00-X, 278 pages.
3. Mikel UV, ed.
Advanced Laboratory Methods in Histology and Pathology.
Washington, DC: Armed Forces Institute of Pathology. 1994;:.
ISBN 1-881041-13-1, 254 pages.