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United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
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This document is provided "as is", without warranty of any kind,
express or implied, including but not limited to the warranties
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PRINCIPLE OF THE TEST.
Coating slides and sections after cutting is performed
for optimal immunohistochemical sections.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
1% Collodion in 100% ethanol.
STEP-BY-STEP DESCRIPTION.
SOLUTION. 1% Collodion in 100% ethanol.
1 gram................. 100ml
1. Deparaffinize slides and bring to 100% ethanol
2. Soak sections in the coating solution for 10 minutes.
3. Drain 1-2 minutes.
4. Immerse in 80% ethanol for 5 minutes.
5. Transfer to water.
6. Stain with appropriate procedures.
REFERENCES.
1. Anderson G, Bancroft J.
Tissue Processing and Microtomy including Frozen. Chapter 6, pp. 85-107.
In: Bancroft JD, Gamble M.
Theory and Practice of Histological Techniques. Fifth Edition.
Edinburgh: Churchill Livingstone. 2002;:85-107.
ISBN 0-443-06435-0, 796 pages.
2. Prophet EB, Mills B, Arrington JB, Sobin LH, eds.
Laboratory Methods in Histotechnology.
Washington, DC: Armed Forces Institute of Pathology. 1992;:.
ISBN 1-881041-00-X, 278 pages.
3. Mikel UV, ed.
Advanced Laboratory Methods in Histology and Pathology.
Washington, DC: Armed Forces Institute of Pathology. 1994;:.
ISBN 1-881041-13-1, 254 pages.