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PRINCIPLE OF THE TEST.
For washing medium in immunohistochemistry.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
Sodium Chloride.
Sodium phosphate (dibasic).
Potassium phosphate (monobasic).
STEP-BY-STEP DESCRIPTION.
SOLUTIONS.
A. Distilled water.................................... 4 liters
B. Sodium Chloride.................................. 27.0 grams, 0.1M
C. Sodium phosphate (dibasic)........................ 7.0 grams
D. Potassium phosphate (monobasic)................... 0.8 grams
1. Add the chemicals in b,c,d,
and adjust the solution with a pH-meter to pH 7.4.
REFERENCES.
2. Woods AE, Stirling JW.
Electron Microscopy: The Preparative Techniques. Chapter 31. pp. 679-700.
In: Bancroft JD, Gamble M.
Theory and Practice of Histological Techniques. Fifth Edition.
Edinburgh: Churchill Livingstone. 2002;:679-700.
ISBN 0-443-06435-0, 796 pages.
2. Prophet EB, Mills B, Arrington JB, Sobin LH, eds.
Laboratory Methods in Histotechnology.
Washington, DC: Armed Forces Institute of Pathology. 1992;:.
ISBN 1-881041-00-X, 278 pages.
3. Mikel UV, ed.
Advanced Laboratory Methods in Histology and Pathology.
Washington, DC: Armed Forces Institute of Pathology. 1994;:.
ISBN 1-881041-13-1, 254 pages.