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United States Government Work, uncopyrighted, public-domain,
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See also: Tissue Stains: 49,
51, 52,
55, 56,
69, 81.
PRINCIPLE OF THE TEST.
To stain paraffinized tissue sections
for mucin, required in the diagnosis of certain diseases
encountered in anatomic pathology,
especially poorly-differentiated adenocarcinomas.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
Weigert's Iron Hematoxylin.
Metanil Yellow Solution.
Mucicarmine Stain.
Absolute ethanol.
95% ethanol.
Xylene or xylene-substitute.
STEP-BY-STEP DESCRIPTION.
FIXATION: 10% Buffered Formalin.
TECHNIQUE: Paraffin. Cut at 6 microns.
SOLUTIONS:
1. Weigert's Iron Hematoxylin
2. Metanil Yellow Solution.
3. Mucicarmine Stain.
STAINING PROCEDURE:
1. Deparaffinize sections, and hydrate to water.
2. Control slide must be used.
3. Place slides in Weigert's hematoxylin for 7
minutes.
4. Wash well in running water. At least 5 minutes.
5. Place slides in Metanil yellow for 1 minute.
6. Wash well in running water.
7. Mucicarmine stain (diluted 1 to 4) for 45
minutes.
8. When satisfactory, wash ftuickly in water.
9. Alcohol - 95% -- 2 changes.
10. Alcohol - 100% -- 2 changes.
11. Xylene -- 2 changes.
12. Mount with poly Mount or any other acceptable
mounting medium.
RESULTS:
Mucin ............... Bright Rose
Nuclei .............. Black
Connective Tissue ... Yellow
THE ABOVE STAINS CAN BE PURCHASED FROM POLY SCIENTIFIC, READY FOR USE.
REFERENCES.
1. Totty BA.
Mucins. Chapter 10, pp. 163-200.
In: Bancroft JD, Gamble M.
Theory and Practice of Histological Techniques. Fifth Edition.
Edinburgh: Churchill Livingstone. 2002;:163-200.
ISBN 0-443-06435-0, 796 pages.
2. Mayer P.
Über Schleimfärbung. [German: On staining mucus].
Mitteil zoolog Station zu Neapel. 1896; 12:303.
3. Mayer P.
Notiz über Hämatin und Hämalaun. [German: Note regarding
Hematin and Hemalaun].
Z f wissenschaftl Mikroskopie u mikroskop Technik. 1903;20:409.
4. Prophet EB, Mills B, Arrington JB, Sobin LH, eds.
Laboratory Methods in Histotechnology.
Washington, DC: Armed Forces Institute of Pathology. 1992;:.
ISBN 1-881041-00-X, 278 pages.
5. Mikel UV, ed.
Advanced Laboratory Methods in Histology and Pathology.
Washington, DC: Armed Forces Institute of Pathology. 1994;:.
ISBN 1-881041-13-1, 254 pages.