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PRINCIPLE OF THE TEST.
To stain paraffinized tissue sections for Acid Fast Bacilli,
required in the diagnosis of certain diseases
encountered in anatomic pathology,
especially tuberculosis and Mycobacterium avium-intracellulare.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
10% buffered neutral formalin.
Paraffin sections at 6 microns.
Xylene-peanut oil solution.
Peanut oil.
Xylene.
STEP-BY-STEP DESCRIPTION.
FIXATION: 10% buffered neutral formalin.
TECHNIQUE: Cut paraffin sections at 6 microns.
SOLUTIONS: XYLENE-PEANUT OIL
Peanut oil............................... 1 part.
Xylene................................... 2 parts.
REFERENCES.
1. Swisher BL.
Microorganisms. Chapter 16, pp. 325-344.
In: Bancroft JD, Gamble M.
Theory and Practice of Histological Techniques. Fifth Edition.
Edinburgh: Churchill Livingstone. 2002;:325-344.
ISBN 0-443-06435-0, 796 pages.
2. Prophet EB, Mills B, Arrington JB, Sobin LH, eds.
Laboratory Methods in Histotechnology.
Washington, DC: Armed Forces Institute of Pathology. 1992;:.
ISBN 1-881041-00-X, 278 pages.
3. Mikel UV, ed.
Advanced Laboratory Methods in Histology and Pathology.
Washington, DC: Armed Forces Institute of Pathology. 1994;:.
ISBN 1-881041-13-1, 254 pages.