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United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
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PRINCIPLE OF THE TEST.
Principles and procedures for quality control
for histology staining procedures.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics, and postmortems,
fresh or in fixative, along with a filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
Distilled water.
10% Neutral buffered formalin.
Paraffin sections at 6 microns.
Absolute ethanol.
Hematoxylin and Eosin Stain.
Control slides for each special stain.
STEP-BY-STEP DESCRIPTION.
1. Hematoxylin and Eosin Stain:
One (1) slide from each tray of slides
is evaluated microscopically after each stain.
2. Special Stains:
There are control slides
for each special stain.
The expected staining results
are listed after each procedure,
and the finished control slides
are evaluated in relation to these expected results.
REFERENCE.
1. Rosai J.
Ackerman's Surgical Pathology. Eighth Edition.
St. Louis: C. V. Mosby. 1996. pp. 2584 - 2585.