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United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
or policies of any United States Government agency.
This document is provided "as is", without warranty of any kind,
express or implied, including but not limited to the warranties
of merchantability, fitness for a particular purpose and
non-infringement. In no event shall the authors be liable
for any claim, damages or other liability, whether in an
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or in connection with the document or the use or other dealings
made with the document.
PRINCIPLE OF THE TEST.
The goal of cell collection and display methods is:
maximal collection area covered with cells; and cells must be
displayed under optimal conditions.
SPECIMEN REQUIRED.
Gynecologic cytology and non-gynecologic cytology specimens,
with a filled-out Tissue Examination Form (SF-515)
for non-gynecologic cytology specimens,
and Gynecologic Cytology Form (SF-541) for gynecologic cytology specimens.
REAGENTS, INSTRUMENTATION.
Microscope. Glass slides. Cytyc data cell processor.
STEP-BY-STEP DESCRIPTION.
1. The goal of all cell collection
and display methods is two-fold.
First, as much of the collection area as possible should be
covered with cells so that the cell sample represents the
original specimen accurately. Secondly, the cells must be
displayed under optimal conditions. These conditions include
such factors as:
a. An even distribution of cells, preferably only one layer.
b. Consistently excellent staining
c. A well mounted permanent preparation containing the
smallest amount of mounting medium practical so that a sharp
microscopic image is obtained.
2. Medical slides prepared in the cytopreparatory laboratory are processed
for optimal cell distribution. The Cytyc ThinPrep Cell Processor
(liquid-based) is used to achieve optimal conditions.
REFERENCES.
1.
Thin-prep Morphology.
Reference Manual, 1994;:2-6.
Cytyc Corporation. pp. 2-6.
2. Rohr LR.
Quality Assurance in Gynecologic Cytology.
Am J Clin Pathol. 1990;94:754-758.