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PRINCIPLE OF THE TEST.
Preparation of alcohol solutions for cytology laboratory.
SPECIMEN REQUIRED.
Gynecologic cytology and non-gynecologic cytology specimens,
with a filled-out Tissue Examination Form (SF-515)
for non-gynecologic cytology specimens,
and Gynecologic Cytology Form (SF-541) for gynecologic cytology specimens.
REAGENTS, INSTRUMENTATION.
95% Ethanol.
Distilled Water.
STEP-BY-STEP DESCRIPTION.
80% ETHYL ALCOHOL (1000 ML):
MIX: 950 ml of 95% ethyl alcohol.
150 ml of distilled water.
70% ETHYL ALCOHOL (1000 ML):
MIX: 750 ml of 95% ethyl alcohol.
250 ml of distilled water.
50% ETHYL ALCOHOL (1000 ML):
MIX: 550 ml of 95% ethyl alcohol.
450 ml of distilled water
(for destaining and restaining).
REFERENCE.
1.
A Manual for Cytotechnology. Fourth Edition.
ASCP, 1975;:262-318.