PAPANICOLAOU STAIN FOR
ROUTINE MEDICAL AND
GYNECOLOGIC CYTOLOGIES.
DRAFT COPY ONLY.
(Procedure 100).
http://www.netautopsy.org/axsop/axsop100.htm


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United States Government Work, uncopyrighted, public-domain, DRAFT COPY ONLY. This document does not necessarily represent the views or policies of any United States Government agency. This document is provided "as is", without warranty of any kind, express or implied, including but not limited to the warranties of merchantability, fitness for a particular purpose and non-infringement. In no event shall the authors be liable for any claim, damages or other liability, whether in an action of contract, tort or otherwise, arising from, out of, or in connection with the document or the use or other dealings made with the document.



PRINCIPLE OF THE TEST.

      Preparation of Papanicolaou stain for routine medical and gynecologic cytologies.



SPECIMEN REQUIRED.
      Gynecologic and non-gynecologic cytology specimens, with Tissue Examination Form (SF-515) for non-gynecologic cytology specimens, and Gynecologic Cytology Form (SF-541) for gynecologic cytology specimens.



REAGENTS, INSTRUMENTATION.
      95% alcohol. 100% alcohol. Gill Hematoxylin I. Lithium carbonate (or Scott's solution). Richard Allen Orange G 6. Richard Allen Eosin A. Clearium mounting medium. Sterile Coplin jars.



STEP-BY-STEP DESCRIPTION.
 

         SOLUTION                  ACTION AND TIME
         95% alcohol               10 Dips (Fixation)
         Tap water                 10 dips 1 minute
         Gill Hematoxylin I        3 minutes
         Running tap water         10 dips
         Bluing                    10 dips
         Running tap water         10 dips
         Cytostain                 1 minute
         95% alcohol               10 dips
         95% alcohol               10 dips
         EA 50                     1 minute
         95% alcohol               10 dips
         95% alcohol               10 dips
         100% alcohol              1 minute
         100% alcohol              1 minute
         100% alcohol              1 minute
         NO XYLENE OR SUBSTITUTE
         Clearium mounting media to cover slip.




REFERENCE.
      1. Bibbo ML.
Comprehensive Cytopathology.
Philadelphia: W.B. Saunders Co. 1991;:881-904.