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United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
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PRINCIPLE OF THE TEST.
To have a written policy for handling highly infectious cases.
SPECIMEN REQUIRED.
Cytology specimens.
REAGENTS, INSTRUMENTATION.
Gown.
Gloves.
Biohazard hood.
Splash shield.
Biohazard bags (red).
Clorox (10%).
50 ml/15 ml centrifuge collection tubes.
Plain glass microscopic slides.
Marking pen.
Pipettes.
Sharps waste container.
STEP-BY-STEP DESCRIPTION.
1. Every specimen received in the Cytology Laboratory
is considered highly infections. Thus universal precautions
are taken. Specimens are received in cytology
in a biohazard transport bag.
Prior to handling specimens,
the processor gowns up and puts on gloves.
A requisition form accompanies the specimen.
The requisition form is taken from the transport bag,
and is assessed for completeness.
If complete, the specimen is accessioned into the VISTA computer system.
2. The specimen is taken from the biohazard transport bag,
and is labeled with an accession number.
The specimen is opened under a biohazard hood,
and allocated into the appropriate capped, numbered centrifuge tubes.
The specimen is centrifuged and the supernatant
of the specimen is poured back into the original container.
The specimen is refrigerated until
it is completely signed out by the pathologist.
Large amounts of unused body fluids are disposed of
by an outside research laboratory, once per week.
3. Sputum container or any other empty containers,
disposable centrifuge tubes, disposable Thin-Prep
filters, disposable gloves, and any other disposable items
used in processing specimens are placed into biohazard bags.
The cytopreparatory technician then takes a solution of 10% Clorox,
and wipes down the table top and the surrounding areas. The bags are removed
from the laboratory, and disposed of the Housekeeping Staff.
4. The cytopreparatory technician discards his/her gloves and gown,
and washes his/her hands.