NEXT PAGE
PREVIOUS PAGE
RETURN TO TABLE OF CONTENTS
United States Government Work, uncopyrighted, public-domain,
DRAFT COPY ONLY. This document does not necessarily represent the views
or policies of any United States Government agency.
This document is provided "as is", without warranty of any kind,
express or implied, including but not limited to the warranties
of merchantability, fitness for a particular purpose and
non-infringement. In no event shall the authors be liable
for any claim, damages or other liability, whether in an
action of contract, tort or otherwise, arising from, out of,
or in connection with the document or the use or other dealings
made with the document.
PRINCIPLE OF THE TEST.
Ordering flow cytometry (as a send out test) on tissue.
SPECIMEN REQUIRED.
All human tissue excised at surgery, outpatient clinics,
fresh or in RPMI fixative; and filled-out Tissue Examination Form
(U. S. Standard Form 515, USSF515).
REAGENTS, INSTRUMENTATION.
§ RPMI fixative.
§ Labeled, sealed specimen container, containing fresh tissue.
§ (U. S. Standard Form 515, USSF515).
STEP-BY-STEP DESCRIPTION.
1.
Cases sent for flow cytometry should be received by the laboratory fresh,
in a container labeled with the patient's identification information
and the tissue site.
2.
Add equal amounts of RPMI medium to the tissue.
RPMI is located in the refrigerator located within the histology laboratory.
3. The specimen and the requisition form should be taken to chemistry
for accessioning as a send out test to be sent to Quest laboratories.
4. Although specimens can be held for up to three days in RPMI,
the quicker the specimen is sent, the greater chance of retaining
viability within cells for flow cytometric analysis.
5. Accessioning should preferably be performed by the
CHEMISTRY LABORATORY SUPERVISOR
or by the CHEMISTRY DIRECTOR.
Additionally, accessioning can be performed by the ordering clinician.
6. The ordering within CPRS is as follows: The test-name should be
ordered as MISCELLANEOUS TEST. The collection sample should be
indicated as BIOPSY.. The specimen type should be indicated as the
specific site or location. The order comment should be indicated
as 5048X, LYMPHOMA FLOW.
7. Results will be placed in CPRS by the chemistry laboratory staff
once a report is received from the reference laboratory.